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Clase: Artigo de Periódico
Título : Evaluation of lugol solution used for counting rumen ciliates
Autor(es): D'Agosto, Marta
Carneiro, Maria Elisa
Resumo: The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
Resumen : -
Palabras clave : Rumen ciliate
Quantitative evaluation
Lugol solution
Technique
CNPq: -
Idioma: eng
País: Brasil
Editorial : -
Sigla de la Instituición: -
Clase de Acesso: Acesso Aberto
DOI: http://dx.doi.org/10.1590/S0101-81751999000300011
URI : https://repositorio.ufjf.br/jspui/handle/ufjf/9180
Fecha de publicación : 1999
Aparece en las colecciones: Artigos de Periódicos



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