Evaluation of lugol solution used for counting rumen ciliates

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Type:	Artigo de Periódico
Title:	Evaluation of lugol solution used for counting rumen ciliates
Author:	D'Agosto, Marta Carneiro, Maria Elisa
Resumo:	The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
Abstract:	-
Keywords:	Rumen ciliate Quantitative evaluation Lugol solution Technique
CNPq:	-
Language:	eng
Country:	Brasil
Publisher:	-
Institution Initials:	-
Access Type:	Acesso Aberto
DOI:	http://dx.doi.org/10.1590/S0101-81751999000300011
URI:	https://repositorio.ufjf.br/jspui/handle/ufjf/9180
Issue Date:	1999
Appears in Collections:	Artigos de Periódicos