Padronização e caracterização de cultura de células tridimensionais de gliobastoma

Abstract

Cancer is one of the leading causes of mortality worldwide, with glioblastoma (GBM) being the most common and aggressive primary brain tumor, characterized by a poor survival prognosis. This study aimed to provide a more accurate platform for investigating cell growth and treatment responses in brain tumors by standardizing and characterizing a three-dimensional (3D) glioblastoma model using U87-MG and T98G cell lines. The liquid overlay technique with agarose was used to prevent cell adhesion and promote spheroid formation. Spheroid growth was monitored over 7 days, assessing diameter and cell viability through the MTT assay, which highlighted the influence of initial cell density on tumor cell behavior. The results showed that the initial cell concentration directly affects the size and viability of the spheroids, with larger spheroids formed at higher concentrations. The 3D model shows potential in simulating the tumor microenvironment, positioning itself as a promising tool for future studies of new treatments for glioblastoma, due to its ability to replicate the cellular interactions of solid tumors.