Expressão das citoceratinas em dermatoses infecto-parasitárias associadas à hiperplasia epidérmica

Abstract

BACKGROUND:Cytokeratins (K) are the major structural proteins of epithelial cells andthey display the greatest heterogeneity of all intermediate filament proteins. The study of manyisolated cytokeratins by immunomarcation enables the structural verification of the cytoskeletonin many neoplastic and inflammatory diseases.OBJECTIVE:To verify the immunohistochemical pattern of cytokeratin expression in infectious andparasitic diseases associated with squamous hyperplasia.METHODS: Histological sections obtained from formalin-fixed and paraffin-embedded tissue fromcromomycosis, paracoccidioidomycosis, leishmaniasis and condylomata acuminata lesions weremarked with the DEK10, LL025, LL002and AE1antibodies by the immunoperoxidase technique (avi-dina-botina).RESULTS:Different degrees of epidermal hyperplasia were observed predominantly or exclusively inthe following four diseases: absence of immunoreactivity to DE-K10in areas of intense epidermalhyperplasia and delayed K10immunohistochemical staining in areas of moderate to discreet/-absent hyperplasia; superbasal expression pattern for K16, regardless of the degree of hyperplasiaas well as superbasal epitops discharging for LL002(C14) and AE1(C10, 14, 16, 19).CONCLUSIONS:The modifications indicate that regardless of the nature of the etiologic agent anddegree of hyperplasia, changes in keratinocyte differentiation and proliferation may occur.