Estudo morfológico do intestino de ratos após a criação cirúrgica de esfíncteres artificiais

Abstract

BACKGROUND: To evaluate variations in weight and diameter of the bowel rats submitted to small bowel surgical sphincter construction comparing values before and after surgery and to study modifications in the mucosal villus. METHOD: Thirty Wistar rats were submitted to the study divided in three groups of ten. Group A (control) - a segment of small bowel of 20mm of extension was removed. Group B - two sphincters, one 100mm and another 150mm from the ileocecal junction were done. Group C - a sphincter from 100mm of the ileocecal junction was done. Bowel samples were collected and villosity studies were studied 10 to 14 days after first surgery. Measurements of bowel diameters and weights were compared before and after surgery modifications at the end of the study. Height villus measurements of the segments on pre and post-sphincters on both Groups B and C and between sphincters in Group B and also intestinal segment of the Group A were done. Data were submitted to statistics analysis using SPSS program. RESULT: Animals weight increased in both groups and in Group B the most. Also, intestine diameter increased and the average of the villus heights was larger. CONCLUSION: Making intestine sphincters results in alterations of the villus layer and increased bowel diameter, similarly to patients that were submitted to wide bowel resections and are adapted. The animals' weight gain can be understood as improvement of nutrients absorption.