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An efficient system for in vitro propagation of Bouchea fluminensis.pdf504.33 kBAdobe PDFThumbnail
Type: Artigo de Periódico
Title: An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae)
Author: Resende, Cristiano Ferrara de
Braga, Virgínia Fernandes
Silva, Cristiane Jovelina da
Pereira, Paula da Fonseca
Ribeiro, Cleberson
Salimena, Fátima Regina Gonçalves
Peixoto, Paulo Henrique Pereira
Resumo: -
Abstract: This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervão-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 µM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 µM). The medium found to induce the greatest number of shoot was that containing 5 µM of BA (NAA-free). For rooting, we evaluated three auxins (NAA, indole-3-acetic acid and indole-3-butyric acid; 0.1, 0.2, 0.3 and 0.4 µM), as well as a control. No differences were observed between the control and the other treatments. The auxin-free medium was deemed the most suitable, because it ensures the lowest cost in the micropropagation procedures. We obtained 100% survival of the acclimatized seedlings, and the plants showed normal vegetative and reproductive development, suggesting that the micropropagation did not alter the biological cycle of this species. The results show the importance and potential of micropropagation for biodiversity conservation of Bouchea fluminensis.
Keywords: Micropropagation
Biodiversity conservation
Espinhaço Mountain Range
CNPq: -
Language: eng
Country: Brasil
Publisher: -
Institution Initials: -
Access Type: Acesso Aberto
Issue Date: Apr-2014
Appears in Collections:Artigos de Periódicos

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