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Detecção de inibidores de proteases em sementes de Punica granatum.pdf1.16 MBAdobe PDFThumbnail
Type: Artigo de Periódico
Title: Detecção de inibidores de proteases em sementes de Punica granatum
Other Titles: Detection of protease inhibitors in seeds of Punica granatum
Author: Colares, Lara Franca
Santos Neto, Antero Ricardo
Cordeiro, Isac Henrique
Castro, Sandra Bertelli Ribeiro de
Verly, Rodrigo Moreira
Alves, Caio Cesar de Souza
Carli, Alessandra de Paula
Resumo: -
Abstract: The use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds.
Keywords: Protease inhibitors
Punica granatum seeds
Traditional medicine
CNPq: -
Language: eng
Country: Brasil
Publisher: -
Institution Initials: -
Citation: COLARES, Lara Franca et al .Detecção de inibidores de proteases em sementes de Punica granatum. Quím. Nova, São Paulo, v. 40, n. 3, p. 270-274, abr. 2017. Disponível em <>. acessos em 03 set. 2018.
Access Type: Acesso Aberto
Issue Date: Apr-2017
Appears in Collections:Artigos de Periódicos

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