Atributos de qualidade associados à ocorrência de proteólise em leite cru granelizado

Abstract

Refrigeration and storage of raw milk at the source of production promoted an improvement in microbial growth, however, the milk obtained in poor hygienic conditions, may cause technological problems through the multiplication of psychrotrophic microorganisms producing extracellular enzymes spoilage, and a lot of times, heat resistant. The objective of this work was to observe the attributes that demonstrate the association with the occurrence of unwanted proteolysis in refrigerated raw milk from Rio Pomba-MG; relating this incident to the development of psychrotrophic contaminants of milk, and beyond to develop and adapt a method capable of detecting light and fast this proteolysis in milk. Observed values as satisfactory physico-chemical characteristics, on the other hand, related to microbiological quality and health values, did not meet the limits set by the legislation mostly. In the study of the correlations, it was concluded that there was an association between total bacterial count, somatic cell count and psychrotrophic in raw milk. And for the values of somatic cell count showed a significant correlation with the percentage of dry extract solids and whey proteins in milk. The amounts related to proteolysis in milk by quantifying tyrosine were significantly correlated with the relative values of the index extension of proteolysis and total bacterial count. And the values of tyrosine by means of quantification kit mucoproteins were observed correlation to total bacterial count and psychrotrophic. In the evaluation test of stability of milk to alcohol 72% v/v. the study of the correlations showed an increase in the index of instability of the samples showed that total bacterial count above 6.04 logCFU.mL-1, and tyrosine above 0.265 mg.5mL-1. Through these results, the test kit proved to be a good indicator as proteolysis occurring in milk, however, the need for further studies on this method, especially on its sensitivity and specificity in order to minimize the influence of other factors to their results.