Avaliação do perfil dos extratos aquoso, etanólico e glicólico de antocianinas obtidos a partir de genótipos de capim elefante com coloração arroxeada

Abstract

This study delves into unraveling the anthocyanin profile of elephant grass hydroalcoholic extracts using capillary electrophoresis and high-performance liquid chromatography. The capillary electrophoresis was unable of detect the anthocyanins due lack of electroosmotic flow, although high performance liquid chromatography allowed the construction of the profile. Besides the extraction process with water and ethanol was optimized through mixture design. Despite higher overall mass extraction efficiency with equal solvent proportions, this doesn't linearly translate to anthocyanin content, hinting at additional compounds in the extracts. Identification of five anthocyanins, with cyanidin-3-glucoside as the most abundant, underscores the extract's complexity. The 25-10:75-90 Water:EtOH ratio emerges with the highest anthocyanin content according to validated polynomial mixture model. A 4-hour extraction optimizes rates, while 18 hours maximizes anthocyanins. These findings hold promise for positively impacting anthocyanin-enriched product production, enhancing nutritional and sensory qualities across diverse industries in agriculture, nutrition, and pharmaceuticals, offering novel avenues for functional and appealing product development.