Caracterização química do esmalte dental humano após infiltração de resina através do fluxo eletrocinético

Abstract

The traditional technique for diffusion of molecules through dental enamel has limitations due to the small size of the pores present in the tissue. In an attempt to optimize the transport of molecules into these pores, the application of electrokinetic flow (ECF) seems to be a promising alternative. Based on a process that generates an electrical potential difference, its application causes movement of the molecules through the enamel nanopores. The objective of this "in vitro" study was to determine the chemical alterations of dental enamel by Fourier Transform Infrared Spectroscopy in the attenuated total reflection mode (FTIR-ATR) after infiltration of an infiltrating resin (Icon Caries Infiltrant Proximal) by FEC. Five third molars were used, which were sectioned longitudinally in the mesio-distal direction, resulting in two hemicrowns. The bands for phosphate, carbonate, protein amide I, C=O bond and for the CH bond were analyzed. (2800-3015 cm−1 ). It was not observed change in the phosphate, amide, and carbonate bands between the infiltrated and non-infiltrated samples. It was also verified the presence of a low intensity band referring to the C=O bond, not seen previously, and an increase in the intensity of the area of the C-H bond in the infiltrated enamel spectrum. The resin was infiltrated into the dental enamel via FEC. However, the vibrational bands presented lower levels than expected, probably due to the small volume of the nanopores in the tissue constitution. The FTIR-ATR method does not seem to have the necessary sensitivity to determine the resin infiltrated into the enamel by FEC.