Caracterização de nanopartículas de titanato de bário e citocompatibilidade em fibroblastos bovinos cultivados in vitro

Abstract

Nanotechnology has been widely used in several areas to improve products' resistance or efficiency, and the demand for this technology in agribusiness has been growing. The nanoparticles of Barium Titanate (NP BaTiO3) are used in biomedicine. However, few studies have evaluated the cytocompatibility and toxicity of these particles in mammalian cells. In particular they are absent in bovine cells. Therefore, this study aimed to evaluate the cytotoxicity of NP BaTiO3 in bovine fibroblasts grown in vitro. The size and Zeta potential of the NP BaTiO3 were evaluated using the dynamic light scattering (DLS) technique, and the morphology was analyzed by scanning electron microscopy (SEM). The cells were cultured in vitro and exposed for 24 hours to different concentrations of NPs to evaluate cell morphology by light microscopy and cytotoxicity using the MTT assay. The data were evaluated by analysis of variance (ANOVA) and the means compared by the Tukey test. Values of P <0.05 were considered significant. The results showed a zeta potential of -13mV, with an average size of 149.267 nm and a polydispersion index of 0.37, with aggregates evidenced by the SEM images. Cell morphology showed no change in any concentration. The MTT test revealed that except for the concentration of 10ug / ml (P <0.05), the NP BaTiO3 did not cause changes in the metabolic activity of the fibroblasts (P> 0.05). In this study’s experimental conditions, the results demonstrated that NP TiBaO3 did not cause cytotoxic effects on bovine fibroblasts and suggest a possible stimulus to cell proliferation at a concentration of 10 ug / ml. However, further studies are needed to assess other toxicity parameters to determine the cytocompatibility of NP TiBaO3.