Isolamento e caracterização de metabólitos secundários das folhas de Xylosma prockia (Turcz.) Turcz. (Salicaceae) e avaliação da atividade antifúngica in vitro frente a linhagens de Cryptococcus spp.

Abstract

The incidence of fungal infections has shown substantial growth in the last decades due to the increase in the immunocompromised population. The species Cryptococcus spp. are responsible for significant systemic infections and have a global estimate of millions of new cases per year. Current therapeutic options are limited and treatments take time and may present a restricted spectrum of action and high toxicity to the patient, which makes the searches for new therapeutic agents an important step in improving the treatments. Previous studies of this research group have shown promising antifungal activity for the polar fractions of the leaves of Xylosma prockia, against Cryptococcus spp. strains. Thus, the present study aimed to characterize the substances present in the ethyl acetate fraction, to identify those responsible for the observed antifungal activity. From this fraction 15 substances were identified: caffeoyl glycoside derivatives (group I), coumaroyl glycoside derivatives (group II) and caffeoyl glycoside derivatives (group III). Four coumaroyl-glycosides were isolated and characterized by spectroscopic methods, three of them was new compounds and one was new for Xylosma genus. The research demonstrates the antifungal potential of ethyl acetate fraction and its subfractions, contributing to the search for new alternatives for the treatment of fungal infections.