Efeito do carvacrol sobre larvas de ​Rhipicephalus microplus ​ (Acari: Ixodidae) em condições seminaturais e citogenotoxidade sobre Brachiaria ruziziensis ​ R.​ Germ & Evrard (Poaceae)

Abstract

The aim of this study was to evaluate an acaricide activity of carvacrol on larvae of Rhipicephalus microplus in leaf blades of Brachiaria ruziziensis and the genotoxicity of this solution on this species of grass. Carvacrol solutions in concentrations of 1,25; 2,50; 5,00; 10,00 e 20,00 mg/mL were sprayed on vessels that had previously received non-fed R. microplus larvae. After a 24-hour application period, the survivors were accounted for and the efficiency of each treatment was calculated. At the lowest concentrations, the efficiency percentages were similar to those of the control, while at the highest concentrations, 10.00 and 20.00 mg/mL, the treatment efficiency was 89.84% and 94.66%, respectively. In the evaluation of genotoxicity of B. ruziziensis , made by flow cytometric analysis, cellular damage up to 100% was observed. Therefore, it is possible to conclude that carvacrol is an alternative in the control of ticks, aiming to reduce the use of chemicals.