Desenvolvimento neonatal de crias de ratas Wistar (Rattus Norvegicus BERKENHOUT, 1769) tratadas com o flavonóide ipriflavona durante a lactação plena

Abstract

The Ipriflavone (7-isopropoxy-3-phenyl-4H-benzopyran-4-one), a synthetic derivative of the isoflavone daidzein, with proven estrogenic activity has been used by women in order to increase bone density and prevent bone loss. Reproductive toxicology studies in the pre-implantation show signs of toxicity, which interferes with implantation of the blastocyst. It was also shown that ipriflavone affects the Hedgehog signaling pathway, important during embryonic development. Studies show that isoflavones are transferred to breast milk which can cause damage to offspring. However no studies were found in toxicology during lactation and development of offspring using the ipriflavone, which is the aim of this work. For the study were used 60 female Wistar rats from the vivarium of the Center for Reproductive Biology, Federal University of Juiz de Fora. The animals were randomly divided into five groups (n = 12) control of the colony, experimental control and treated 1, 2 and 3. The first group did not have any type of manipulation and was used to assess the possible effects of gavage. The second group received intragastrically, twice a day, from the 2nd to the 16th days of lactation, 1 ml of distilled water and the treated groups received, by the same procedure, 1 ml of water suspension of ipriflavone at doses of 0,01; 0,05 e 0,1 mg/g/day, respectively. Maternal variables were analyzed: presence of clinical signs of toxicity, body weight gain and food intake. Maternal behaviors were observed as: breastfeeding posture, organization and maintenance of the nest, the act of recovering, collecting pups and licking them. The pups were assessed for physical development and reflexology. As physical variables were analyzed: body weight, eye opening, unfolding of the ears, and appearance of lanugo hair, eruption of upper and lower incisors, the vaginal opening or descent of the testicles. For observing the reflexology development the following tests were made: grip, posture response, dodging the abyss, orientation, and negative geotaxis. In the experimental model studied ipriflavone showed no signs of maternal toxicity, or changes in the physical and reflexology development of offspring.