Avaliação do efeito imunomodulador da isoflavona genisteína na encefalomielite autoimune experimental murina

Abstract

Multiple sclerosis (MS) is the most common non-traumatic, disabling neurological human inflammatory demyelinating disease of the CNS. Experimental autoimmune encephalomyelitis (EAE) models MS and is characterized as a CD4+ Th1 cell-mediated autoimmune disease. Recent data show that estrogen plays a pivotal role in the immune response as well as in immune-mediated diseases. Isoflavones are found in a variety of plants, especially in soy, and make up the most common form of phytoestrogens which demonstrate estrogenic properties due to their structural similarities with estrogens. Genistein is the major bioactive isoflavone. In the present report, we investigated the use of genistein for the treatment of the murine model of MS in C57Bl/6 mice. The genistein’s modulatory effects were evaluated after induction of EAE with MOG35-55, assessing not only clinical symptoms but also pro- and anti-inflammatory cytokines, and the leukocyte rolling and adherence in the CNS by performing intravital microscopy.