Desenvolvimento fetal em ratas wistar tratadas com ipriflavona durante a fetogênese

Abstract

The ipriflavone is a synthetic derivative of the isoflavone daidzein, marketed with the aim of preventing and / or treating osteoporosis. It was verified in vitro that ipriflavone (IP) induced apoptosis in gastric epithelial cells and inhibited proliferation and DNA synthesis of bone tumor cells. The daidzein, the precursor of ipriflavone, had anti-implantation effect in rats and reduced production of progesterone in human trophoblast cells. Genistein is structurally similar to ipriflavone, has harmful effects on the blastocysts of mice, cultured in vitro and in vivo. The fetogenesis is a period of embryonic development in which there is intense mitosis and cell proliferation, which characterize the growth and differentiation of various organ systems, finishing the formation of reproductive system and central nervous systems of final development of the limbs and face. All these processes can be altered by the effects of iproflavone, but there are no studies to assess them, the purpose of this study was to evaluate fetal development in rats treated with aqueous solution of Ipriflavone during the fetogênese. 60 rats were used at 10 days post-coitus (pc), maintained under controlled temperature, humidity and ventilation, which were randomly distributed in one control group - C (1 ml of distilled water) and three treated groups T1, T2 and T3 that received, respectively, 300, 1500 and 3000mg/kg of ipriflavone, intragastric, from 16 to 20 pc. The animals were euthanized at 21º days pc. Variables analyzed: maternal organ weights (liver, kidney, supra kidney, spleen and ovary), length and fetal body weight, weight of placenta, brain, liver, kidney and lung. Statistics gathering data analysis: ANOVA followed by Dunnett test (α = 0.05). The experimental procedure was approved by the Ethics Committee on Animal Experimentation of Federal University of Juiz de Fora (Protocol No. 024/2008). Treatment with aqueous suspension of ipriflavone caused no significant difference in any of the variables studied. We can conclude that in the experimental model used, the ipriflavone did not cause maternal toxicity and significant changes in fetal development.