Protocolos de descelularização da polpa dentária

Abstract

The aim of the present study was to review the literature about the existing decellularization protocols to obtain a scaffold through the extracellular matrix (ECM) of the dental pulp. Decellularization is a process that aims to transform an organ or tissue into a scaffold to receive new cells and initiate a regenerative process. It can be performed with agents and/or methods used alone or in combination, with the aim of achieving a scaffold with a smaller number of cells, including DNA and immune cells, in addition to preserving essential components and structures of the ECM. A search was carried out for scientific articles in the Pubmed database, within the period from 2008 to 2022, on dental pulp and its decellularization protocols. The reviewed literature made it possible to conclude that there are several protocols that use different agents and methods in different concentrations and time, but succeeding in terms of their respective efficiencies. However, they also highlighted the absence of a standardized protocol and the need for it, in addition to the difficulties imposed by the clinical reality.