Desenvolvimento e validação de metodologias analíticas para quantificação de β-escina em extratos de Aesculus hippocastanum L. (Castanha da Índia)

Abstract

All people around the world use medicinal plants, or its derivatives, for disease’s treatment. The herbal medicines are obtained by using, as an active principle, only raw vegetables and characterized by knowledge of the effectiveness and risks of its use. In Brazil, these products are regulated by the Agência Nacional de Vigilância Sanitária (ANVISA) and shall provide criteria for quality, safety and efficacy required. The β-escin is the major active proved from seeds of Horse-Chestnut, Aesculus hippocastanum (Hippocastanaceae) that has pharmacological properties that produce significant responses in cases of chronic venous insufficiency (CVI), hemorrhoids and edema postoperatively. As a product for pharmaceutical use is widely used and has several presentations, on various pharmaceutical forms. The Horse-Chestnut (Aesculus hippocastanum L.) is the second plant species with the highest number of derivatives recorded as simple herbal medicine at ANVISA. The lack of analytical methodology for quantification of β-escin in fluid extracts, glycolic and dry of Horse-Chestnut prompted the need for development of this study whose aim was to develop and validate analytical methods for determination of this substance. We developed three different analytical methodologies using UV visible spectrophotometer, High Performance Liquid Chromatography and Capillary Electrophoresis. The fluid extract was analyzed by three techniques validated. The methods were compared using Student's t test and showed statistical differences in the 95% confidence. The confidence interval of the methods was also calculated, which are 0.16 to 0.73, 0.21 to 0.87 and 0:24 to 1:15, respectively, for HPLC, CE and UV-Vis. The amount declared by the manufacturer was 0.47%, while this value in the confidence interval of all methodologies.