Estudos de novas tinturas mãe homeopáticas de plantas medicinais da região de Governador Valadares-MG

Abstract

Mother tinctures (MTs) are ethanolic extracts obtained from plants, being a type of formulation frequently used in homeopathic production of health problems. Its therapeutic effect is partly attributed to the presence of phytochemicals and is often associated with popular knowledge. In this paper, thirteen mothers tinctures were studied, obtained from parts (leaf, root, bark, rhizome, flower) of nine different plants collected in Governador Valadares- MG region. The MTs were prepared by fresh plant material maceration of the following plants: Petiveria alliacea (Guiné); Solidago chilensis (Arnica); Erythrina verna (Mulungú); Myrciaria dubia (Camu-camu); Manilkara longifolia (Paraju); Pfaffia glomerata (Ginseng- brasileiro); Costus spicatus (Cana de Macaco); Spondias tuberosa (Umbu); Curcuma longa (Açafrão da terra), in hydroalcoholic solution 70%. The MTs biological activity was evaluated against cell viability with dimethylazole diphenyltetrazolium bromide (MTT) and nitric oxide (NO) production in murine macrophages (RAW 264.7) after the development of lipopolysaccharide (LPS ) and Interferon-gamma (IFN-γ). In addition to the content of total phenolic compounds (CPT) by the Folin-Ciocalteu method and the antioxidant activity (AA) using free radicals DPPH (2,2-diphenyl-1-picrylhydrazyl). In general, MTs did not show cytotoxicity to RAW 264.7 cell culture. All extracts inhibited NO production at some tested concentration (2.5, 5.0, 10.0 μg / mL), but the Paraju (leaf), Umbu (leaf) and Safflower (Rizoma) extracts had the best. The total phenolic compounds content of the plants ranged from 41.5 - 1449.1 mg of gallic acid (GAE)/L mother tincture. The MTs with higher content of phenolic compounds were Camu-camu (leaf) and Paraju (leaf). And antioxidant activity in terms of IC50 varied between 14.1 - 445.8 mg GAE / L mother tincture. The MTs with better antioxidant activity are Ginseng-Brazilian (rhizome), Paraju (leaf). The importance of the presence of phenolic compounds with antioxidant potential observed in all the MTs studied is highlighted in this paper. In the same sense, some MTs studied also showed a capacity to inhibit NO production, making promising samples for further studies.