Ocorrência de Aggregatibacter actinomycetemcomitans na saliva total em pacientes sob tratamento ortodôntico

Abstract

Orthodontic treatment has potential to cause changes in periodontal due to biofilm accumulation, due to the difficulty of conducting a proper oral hygiene in the presence of bands and brackets. OBJECTIVES: Evaluate the occurrence of Aggregatibacter actinomycetemcomitans in whole saliva in individuals before and after the installation of metal orthodontic braces and assess whether there has been deterioration of periodontal status (visible plaque index, probing depth and bleeding on probing) after installing braces. METHODS: We evaluated 15 patients of Specialization in Clinical Orthodontics, School of Dentistry - UFJF and private practices, female and male, with an average age of 18 years and 3 months (14-18 years); 7 men and 8 women. Whole saliva samples were collected one month before (T1), immediately before (T2), 15 days before (T3) and one month after the installation of fixed Edgewise Standard unit (Morelli 10.30.900) on the upper and lower teeth, except the second and third molars. The saliva collection was done with the Salivete Cortisol device (Sarstedt ® Nümbrecht, Germany), with neutral swab polyester and micro tube. From samples collected, extraction of DNA for bacteria identification was carried out (Aa) by Polymerase Chain Reaction method in real time. RESULTS: After the analysis, there was no presence of bacteria Aggregatibacter actinomycetemcomitans in any of the intervals of the samples tested. There was no deterioration of the periodontal status (visible plaque index, probing depth and bleeding on probing) after T3. CONCLUSIONS: a) Unable to identify instances of Aa bacteria in saliva either before or after bonding of fixed appliances; b) the use of braces did not contribute to increase plaque index, probing depth and bleeding on probing.