Estratégias de pesquisa para identificação do mecanismo de ação de antimicrobianos

Abstract

Although bacteria can naturally develop antimicrobial resistance, antibiotic resistance has become a crisis, leading us to seek more and more antimicrobial alternatives for medical practice and therapy. In the face of this cruel reality, the present work sought to present a review of laboratory methods that are easily applicable and that evaluate the mode of action of different types of compounds, since this is a prerequisite for the development and discovery of new drugs, contributing, then, in the search for new antimicrobial agents. The literature was screened using the keywords "antibiotic", "mode of action", "antimicrobial activity", "method", "bacteria" and "mode of inhibition" in the databases Pubmed, Scopus, BVS and Science Direct, with works published between 2000 and 2019. This review sought to demonstrate the wide range of methods used in the search for mechanisms of action of antibacterial substances, in addition to bringing the experimental protocols available in the literature related to the techniques covered. The research and discovery of new drugs brings optimism towards new therapeutic alternatives in the future. This review is expected to make it possible to start the experiments in a more practical and quick way.