Avaliação do potencial cicatrizante do extrato em acetato de etila 2% e 5% de Cecropia pachystachya em lesão cutânea de ratos Wistar

Abstract

The use of medicinal plants is part of History, which is of great importance to promote medical treatment of various diseases. The plant kingdom has significant contribution as providers of useful substances to humans. The variety and complexity of plant metabolites plays a key role in biological activities. There are reports that Cecropia pachystachya presents anti-inflammatory, antitussive, anti-asthmatic, antihistamine, antispasmodic, expectorant, hypoglycemic, diuretic, tonic, antihemorragica, astringent, hypotensive and wound healing activities. Thus, the present study aimed to evaluate the antioxidant activity of ethyl acetate extract of the leaves of C. pachystachya and the wound healing activity of a gel preparation containing this extract. Moreover, the chromatographic profile of the extract was determined by HPLC. Antioxidant activity was evaluated by DPPH, reducing power and TBA assays. The wound healing activity was evaluated in vivo by open wounds created surgically in rat skin, which were treated with vehicle (Carbopol gel 1%) or gel containing 2% or 5% C. pachystachya extract (ECP) and evaluated macroscopically and microscopically after 3, 7, 14 or 21 days. It was observed early inflammation reduction in the group treated with ECP 5% after 3 days. After 21 days, all groups showed similar results, although the groups treated with 2% and 5% ECP showed better tissue organization in the repair process. The extract showed remarkable antioxidant activity, probably due to the presence of flavonoids such as orientin and isoorientina identified in the extract, which may be related to the wound healing activity found for this plant.