Avaliação da atividade antifúngica da fração acetato de etila das folhas de xylosma prockia frente a candida parapsilosis

Abstract

Candida parapsilosis is among the most common etiological agents of candidemia, with a percentage of 19% of cases in Brazil. The therapy available for the treatment of candidiasis is limited due to high toxicity and high fungal resistance to clinically available drugs. Xylosma prockia (Turcz.) Turcz. is a plant native to Brazil with little information about its pharmacological potential. In this work, the in vitro antifungal effects of the ethyl acetate fraction of X. prockia leaves were evaluated against C. parapsilosis (ATCC 22.019). The death curve test showed that the fraction has a fungicidal character and is time-dependent. In the quantification of ergosterol, it is observed that the fraction does not interfere with the synthesis of the steroid. The lipid peroxidation assays and quantification of reactive oxygen species and nitrosative species showed that the fraction causes cell membrane peroxidation and nitrosative damage by inducing reactive nitrogen species, respectively. The results of this study provide evidence that the ethyl acetate fraction of X. prockia leaves can indeed be a potential source of substances with antifungal potential.