Rugosidade superficial de diferentes bráquetes ortodônticos e sua relação com a adesão de streptococcus mutans – estudo in vitro

Abstract

Orthodontic treatment alters the oral ecology by introducing new retentive areas for microbial colonization, being associated with high levels of Streptococcus mutans. The surface characteristics of the brackets may affect bacterial adhesion and bacterial colonization. The study aims to characterize the surface roughness of different types of orthodontic brackets and to relate them to the adhesion of S. mutans. The groups were divided into MSL (self-ligating metallic), C (ceramic aesthetics) and CM (conventional metallic). The surface areas of the brackets were defined in 3D scanning and subsequently subjected to surface roughness reading. Bacterial adherence was evaluated after 8h and 24h of bacterial growth in BHI broth supplemented with sucrose (1%) by the biofilm formed views (Scanning Electron Microscopy - SEM), total cell quantification (optical density reading - OD) and quantification of viable cells (colony forming units - CFU). The ceramic bracket presented significantly higher surface roughness than the other evaluated brackets. However, this difference in roughness was not reflected in bacterial adhesion, which was not significant considering OD and CFU. The ceramic bracket had higher surface roughness, however, there was no significant difference in the levels of bacterial adhesion between the evaluated brackets.